Tabriz University of Medical Sciences Health Promotion Perspectives 2228-6497 13 3 2023 09 11 Diversity of Bacteroidaceae family in gut microbiota of patients with chronic kidney disease and end stage renal disease 237 242 10.34172/hpp.2023.29 EN Siamak Amini Khiabani https://orcid.org/0000-0002-7146-5652 Setareh Haghighat https://orcid.org/0000-0002-6605-5245 Hamid Tayebi Khosroshahi https://orcid.org/0000-0002-1131-0413 Mohammad Asgharzadeh https://orcid.org/0000-0001-7376-929X Hossein Samadi Kafil https://orcid.org/0000-0001-6026-8795 Journal Article 10.34172/hpp.2023.29 2023 07 18 2023 07 29 Background: Human intestine microbiota are known to be directly and indirectly altered during some diseases such as kidney complications. Bacteroides is considered as the main and the most abundant phylum among human gut microbiota, which has been classified as enterotype 1. This study aimed to assess the abundance of Bacteroides spp. in fecal flora of end-stage renal disease (ESRD) and chronic kidney disease (CKD) patients and compare it with the Bacteroides composition among fecal flora of healthy individual. Methods: Fresh fecal samples were collected from 20 CKD/ESRD patients and 20 healthy individual without any kidney complications. The pure microbial DNA was extracted by QIAamp Stool Mini Kit from stool samples. MiSeq system was used to analyze the intestinal composition by next generation sequencing method. Results: A number of 651 bacterial strains were isolated and identified from 40 fecal samples of both patients and healthy groups. Bioinformatics analysis defined 18 different types of Bacteroides species which included 2.76% of all strains. Statistical analysis showed no significant difference between study groups (P>0.05). In both healthy and patient groups three species including B. dorei, B. uniformis, and B. ovatus have allocated the most abundance to themselves. The lowest abundance was related to B. eggerthii, A. furcosa and B. barnesiae among CKD/ESRD patients and A. furcosa, B. barnesiae, and B. coprocola had the lowest abundance among healthy people. Conclusion: This study indicates despite all previous evidence of Bacteroides role in gut microbiota, it had no different distribution between healthy persons and CKD/ESRD patients.